Mahmood Rasool*, Arif Malik, Mubashir Hussain, Kalsoom A. Haq, Kiran Butt, Muhammad Abdul Basit Ashraf, Muhammad Imran Naseer, Muhammad Asif, Rozena Shaikh, Mohammad Zahid Mustafa, Qamre Alam, Ghulam Rasool, Waseem Ahmad, Absarul Haque and Mohammad Amjad Kamal Pages 1610 - 1615 ( 6 )
The therapeutic significance of bioengineering proteins has increased dramatically as a new generation of pharmacological drug with a great potential in medical treatment. Protein engineering has improved the use of new non immunoglobulin affinity proteins. The designed ankyrin repeat proteins abbreviated as DARPins is a classical example. These molecules have smaller structural size, unlike immunoglobulins, but similar target affinity is of great significance. Such molecules get more binding specificity and greater thermodynamic stability. These attributes made DARPins a favorite player in diagnostics and therapeutics of cancer. How DARPins are improved into vigorous and versatile scaffold for binding protein and selected by ribosome and phage display along with related aspects is discussed in this review.
Designed ankyrin repeat proteins, Protein bioengineering, Therapeutics.
Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Institute of Molecular Biology and Biotechnology, (IMBB), The University of Lahore, Lahore, Institute of Molecular Biology and Biotechnology, (IMBB), The University of Lahore, Lahore, Institute of Molecular Biology and Biotechnology, (IMBB), The University of Lahore, Lahore, Institute of Molecular Biology and Biotechnology, (IMBB), The University of Lahore, Lahore, Institute of Molecular Biology and Biotechnology, (IMBB), The University of Lahore, Lahore, Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences (BUITEMS), Quetta, Department of Biotechnology, Balochistan University of Information Technology, Engineering and Management Sciences (BUITEMS), Quetta, CASVAB, University of Balochistan, Quetta, King Fahd Medical Research Center (KFMRC), King Abdulaziz University, Jeddah, Department of Plant Breeding and Genetics, Faculty of Agriculture, Ghazi University, Dera Ghazi Khan, Center of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, King Fahd Medical Research Center (KFMRC), King Abdulaziz University, Jeddah, Enzymoic, 7 Peterlee Place, Hebersham, NSW 2770