Sinem Engin, Dagmar Fichtner, Doris Wedlich and Ljiljana Fruk Pages 5443 - 5448 ( 6 )
SNAP-tag technology has been an important tool for protein study for more than a decade and in the meanwhile has found a number of applications beyond the field of molecular biology and protein purification. Based on covalent interaction of SNAP-tag, 20 kDA mutant of DNA repair protein and benzylguanine, it enables irreversible and controllable protein modification. In this mini review, recent developments in the use of SNAP-tag for the design of protein arrays and nanoparticle biofunctionalization are presented and discussed. A short overview of other applications that paved the way to surface modifications is also given with emphasis on fluorescent labeling through the use of SNAP-tag fusion proteins. Finally, the future of the SNAP-tag methodology for surface patterning and 3D structural scaffolding is addressed.
SNAP-tag, fusion proteins, benzylguanine, micro-contact printing, protein-nanoparticle conjugation, DNA directed immobilization, DNA origami.
, , , Karlsruhe Institute of Technology (KIT), DFG-Center for Functional Nanostructures (CFN), Wolfgang Gaede Str. 1, 76131 Karlsruhe, Germany.