Daniela Jara-Moreno, Ana L. Riveros, Andrés Barriga, Marcelo J. Kogan* and Carla Delporte* Pages 1365 - 1376 ( 12 )
The β-amyloid peptide (1-42) is a molecule capable of aggregating into neurotoxic structures that have been implicated as potential etiological factors of Alzheimer’s Disease. The aim of this study was to evaluate the inhibition of β-amyloid aggregation of ethyl acetate and ethanolic extracts obtained from Ugni molinae leaves on neurotoxic actions of β-amyloid aggregates. Chemical analyses were carried out with the extracts in order to determine their phenolic profile and its quantification. Both extracts showed a tendency to reduce neuronal deaths caused by β-amyloid. This tendency was inversely proportional to the evaluated concentrations. Moreover, the effect of EAE and ETE on β-amyloid aggregation was studied by fluorimetric T Thioflavin assay and transmission electronic microscopy (TEM); the extracts showed a modulation in the aggregation process. Partly, it is believed that these effects can be attributed to the polyphenolic compounds present in the extracts.
Ugni molinae, β-amyloid aggregation, T Thioflavin, polyphenols, β-amyloid disaggregation, fluorimetric.
Laboratorio de Productos Naturales, Departamento de Quimica Farmacologica y Toxicologica, Facultad de Ciencias Quimicas y Farmaceuticas, Universidad de Chile. Sergio Livingston 1007, Santiago 8380492, Laboratorio de Nanobiotecnologia y Nanotoxicologia, Departamento de Quimica Farmacologica y Toxicologica, Facultad de Ciencias Quimicas y Farmaceuticas, Universidad de Chile. Sergio Livingston 1007, Santiago 8380492, Unidad de espectrometria de masas, Facultad de Ciencias Quimicas y Farmaceuticas, Universidad de Chile. Sergio Livingston 1007, Santiago 8380492, Laboratorio de Nanobiotecnologia y Nanotoxicologia, Departamento de Quimica Farmacologica y Toxicologica, Facultad de Ciencias Quimicas y Farmaceuticas, Universidad de Chile. Sergio Livingston 1007, Santiago 8380492, Laboratorio de Productos Naturales, Departamento de Quimica Farmacologica y Toxicologica, Facultad de Ciencias Quimicas y Farmaceuticas, Universidad de Chile. Sergio Livingston 1007, Santiago 8380492